Dr. John DeStafeno, M.D. is a board-certified ophthalmologist specializing in all aspects of refractive cataract surgery and laser surgery as well as the treatment of complex corneal diseases. He completed his 2-year fellowship at Duke University which focused on clinical research and the medical and surgical management of anterior segment disease.
Dr. John DeStafeno, M.D. is a board-certified ophthalmologist specializing in all aspects of refractive cataract surgery and laser surgery as well as the treatment of complex corneal diseases. He completed his 2-year fellowship at Duke University which focused on clinical research and the medical and surgical management of anterior segment disease.
Dr. John DeStafeno, M.D. is a board-certified ophthalmologist specializing in all aspects of refractive cataract surgery and laser surgery as well as the treatment of complex corneal diseases. He completed his 2-year fellowship at Duke University which focused on clinical research and the medical and surgical management of anterior segment disease.
Dr. DeStafeno has brought several innovative medical and surgical treatments to our area and is an experienced all-laser LASIK surgeon. In addition, Dr. DeStafeno was the first surgeon in Chester County to perform DSAEK corneal transplantation.
Recognized both locally and nationally for his excellence in eye care, Dr. DeStafeno has received numerous Top Doctor and Surgeon Awards. For the past several years, he has been selected by peers to instruct the LASIK Surgery Course at the annual American Academy of Ophthalmology meeting. Dr. DeStafeno also enjoys educating the future of eye care, instructing ophthalmology residents at Wills Eye Hospital at Thomas Jefferson.
EDUCATION
Graduated Summa Cum Laude, Siena/Albany Medical College Program in Science and Humanities, Albany, NY
Doctor of Medicine degree from Albany Medical College
Residency in Ophthalmology at Long Island Jewish Hospital, Albert Einstein College of Medicine, serving as chief resident senior year
Two-year Clinical/Research Fellowship in Cornea, Refractive, and External Disease at the Duke University Eye Center, Duke College of Medicine
POSITION AND DISTINCTIONS
Attending Surgeon at the Chester County Hospital
Certified Intralase LASIK surgeon
Certified Wavelight LASIK surgeon
Clinical Instructor for residents at Wills Eye Hospital/Thomas Jefferson University Hospital
Founding member Vanguard Ophthalmology Society
Instructor, LASIK Surgery Course, American Academy of Ophthalmology
AWARDS AND HONORS
American Academy of Ophthalmology Achievement Award
Top 300 Refractive Cataract Surgeons by Ocular Surgery News
Top Doctor in Main Line Today magazine
Top Doctor in Philadelphia magazine
Top 250 Premium IOL Surgeons in Premier Surgeon magazine
Alpha Omega Alpha Medical Honor Society
PERSONAL SNIPPET
Originally from New York, Dr. DeStafeno has grown to love Chester County and the Philadelphia region. He enjoys supporting local community arts, festivals, and sporting events with his wife and three children. He also loves playing tennis and traveling with his friends and family.

Couldn’t be happier with the services provided. Totally professional and top notch. Friendly staff Couldn’t be more pleased! The Best
As far as the Doctor goes very happy. As far as he staff being the ones that did the testing on me, I thought they lacked personality and seemed like this is just a job and really treated you like you were just another patient And let’s get this over with…
Dr DeStafeno and his entire team are amazing!!! Would highly recommend them!!
He was very thorough. explained everything step by step. He did a terrific job. Would recommend him highly.
The doctor was very efficient. Gave a thorough explanation of the procedure at my pre op appointment. He answered my questions so that I had a good understanding of cataract surgery. He visited me in the pre op area both surgeries for short explanation of the steps. The anesthesiologist also visited me both times in the pre op area. All the nursing staff were very kind and helpful and, also, very efficient. I’m still in the healing process but, so far, my outcome is wonderful.
Takes time to explain procedures in easy to u destined terms.
So thorough with his examination - makes me feel very secure about my eyesight. Thank you.
Doctor DeStafeno pleasant easy to understand explains everything Waite time was nothing more than any ither but on time
He took the time to fully explain my condition and the path forward.
Dr. DeStafeno is the best. I moved to NJ but I drive back to see him because he’s so good.